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  1. Preparation Of Buffer Solutions (Phosphate, Acetate And Different Buffers)

    Acetic Ammonia Buffer pH three.7, Ethanolic: To 15 ml of 5 M acetic acid add 60 ml of ethanol (ninety five p.c) and 24 ml of water. Regulate the pH to three.7 with 10 M ammonia and dilute with water to one hundred ml. Acetone Resolution, Buffered: Dissolve 8. 15 g of sodium acetate and 42 g of sodium chloride in water, add 68 ml of 0.1 M hydrochloric acid and 150 ml of acetone and dilute with water to 500 ml. Buffered Saline pH 7.2: Dissolve 10.Seventy five g of disodium hydrogen phosphate, 7.6 ...