Store in polyethylene containers. Ammonia Buffer pH 10.Zero: Dissolve 5.4 g of ammonium chloride in 20 ml of water, add 35 ml of 10 M ammonia and dilute with water to one hundred ml. Ammonia Buffer pH 10.9: Dissolve 67.5 g of ammonium chloride in enough 10 M ammonia to produce 1000 ml. Barbitone Buffer pH eight.6, Combined; Barbitone Buffer pH 8.6: Dissolve 1.38 g of barbitone, 8.76 g of barbitone sodium and 0.38 g of calcium lactate in adequate water to supply 1000 ml. ] M sodium hydroxide (about ...
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